Journal: Scientific Reports

Article Title: Porphyromonas gingivalis promotes oral squamous cell carcinoma progression via the IL-6/EZH2/Snai2 axis

doi: 10.1038/s41598-026-41528-w

Figure Lengend Snippet: Exploration of the functions of EZH2 and Snai2 in the OSCC progressions. (A) EZH2 and Snai2 expression level in primary OSCC cells (POSCC) or normal oral epithelial cells as control (NC) before and after IL-6 treatment. (B) EZH2 and Snai2 mRNA transcription level in primary OSCC cells (POSCC) before and after IL-6 treatment. (C) Cell proliferation assay of the OSCC cell line, HSC-2, with different treatments targeting EZH2. EZH2-OE: EZH2 overexpression; EZH2-KD: EZH2 knockdown; EZH2-KD & ReEZH2: EZH2 knockdown cells with EZH2 recombinant protein rescuing management. (D) Cell migration assay of HSC-2 with different treatments targeting EZH2. The cell migration rate was monitored by RTCA analyzer and calculated as cell index. (E) mRNA transcription level of Snai2 in HSC-2 with different treatments targeting EZH2. (F) Cell proliferation assay of HSC-2 with different treatments targeting Snai2. Snai2-OE: Snai2 overexpression; Snai2-KD: Snai2 knockdown. (G) Cell migration assay of HSC-2 with different treatments targeting Snai2. Each experiment for RTCA analysis was performed with duplication of samples ( N = 2), while for other items was with triplication of samples ( N = 3) and the error bars were presented as SD result. The independent experiment was performed at least three times. For the statistic test using, results in panel B were analyzed with student’s t-test, in panel C, E and F were analyzed with One-way ANOVA method, and in D and G were with Two-way ANOVA method.

Article Snippet: Samples were blocked with 5% non-fat milk in TBST for 1 h at room temperature and incubated with the EZH2 primary antibody (1: 1000, orb1939414, biorbyt, UK) and Snai2 primary antibody (1: 1000, orb197797, biorbyt, UK) overnight at 4 °C.

Techniques: Expressing, Control, Proliferation Assay, Over Expression, Knockdown, Recombinant, Cell Migration Assay, Migration

Journal: Scientific Reports

Article Title: Porphyromonas gingivalis promotes oral squamous cell carcinoma progression via the IL-6/EZH2/Snai2 axis

doi: 10.1038/s41598-026-41528-w

Figure Lengend Snippet: Determination of the effects of EZH2 regulated Snai2 signal on OSCC progression. (A) Wound healing images (left panel) and the quantification data (right panel) of HSC-2 from 0 h to 24 h, in different groups. EZH2-OE: EZH2 overexpression; SiSnai2: SiRNA treatment targeting Snai2. (B) Cell proliferation assay of HSC-2 with different treatments. (C) Cell migration assay of HSC-2 with different treatments. The cell migration rate was monitored by RTCA analyzer and calculated as cell index. (D) Cell proliferation assay of POSCC with different treatments. POSCC: primary OSCC cells without treatment; IL-6: primary OSCC cells with IL-6 stimulation; IL-6 & inEZH2: primary OSCC cells with EZH2 inhibitor treatment after IL-6 stimulation; IL-6 & SiSnai2: primary OSCC cells with Snai2 SiRNA treatment after IL-6 stimulation. (E) Cell migration assay of POSCC with different treatments. Each experiment for RTCA analysis was performed with duplication of samples ( N = 2), while for other items was with triplication of samples ( N = 3) and the error bars were presented as SD result. The independent experiment was performed at least three times. For the statistic test using, results in panel A, B and D were analyzed with One-way ANOVA method, and in C and E were with Two-way ANOVA method.

Article Snippet: Samples were blocked with 5% non-fat milk in TBST for 1 h at room temperature and incubated with the EZH2 primary antibody (1: 1000, orb1939414, biorbyt, UK) and Snai2 primary antibody (1: 1000, orb197797, biorbyt, UK) overnight at 4 °C.

Techniques: Over Expression, Proliferation Assay, Cell Migration Assay, Migration

Journal: Scientific Reports

Article Title: Porphyromonas gingivalis promotes oral squamous cell carcinoma progression via the IL-6/EZH2/Snai2 axis

doi: 10.1038/s41598-026-41528-w

Figure Lengend Snippet: Bioinformatic analysis of relationships among Snai2, EMT, and P.g. infection. (A) GSEA enrichment of Snai2 in OSCC correlating with the terms from Biological Process (right panel), Molecular Functions (mid panel), and Cellular Structures (left panel). (B) Volcano plot of the DEGs in OSCC samples standardized to the normal samples. (C) The Kaplan–Meier (K-M) survival curve of OSCC patients with Snai2 expression. The x-axis and y-axis were pointing time and survival probability, respectively, and the high Snai2 expression group and low Snai2 expression group were illustrated as red line and blue line, respectively. D , E. Functional enrichment of the screened DEGs in KEGG database with the Benjamini & Hochberg analyzing method. Relevant signal pathways (D) and their relationships with IL-6 and Snai2 (E) were shown. F. PPI analysis of the DEGs corresponding proteins in the selected genes group from KEGG enrichment. The selected proteins were divided to two groups, the infection relevant proteins (orange) and the cancer metastasis relevant proteins (teal). The proteins IL-6, EZH2 and Snai2 were highlighted with green color.

Article Snippet: Samples were blocked with 5% non-fat milk in TBST for 1 h at room temperature and incubated with the EZH2 primary antibody (1: 1000, orb1939414, biorbyt, UK) and Snai2 primary antibody (1: 1000, orb197797, biorbyt, UK) overnight at 4 °C.

Techniques: Infection, Expressing, Functional Assay